Skip to main content
. 2020 Dec 18;2(1):100230. doi: 10.1016/j.xpro.2020.100230

Figure 1.

Figure 1

Exclusive labeling of Cre-positive and -negative cells by an AAV vector

(A) Construct of the AAV vector. The vector expresses RFP or GFP in the absence or presence of Cre recombinase by a flip-excision (FLEX) switch. Both GFP and RFP are tagged with a membrane-targeting protein, palmitoylation signal (pal).

(B) Schematic diagram of the exclusive labeling method. Two cell populations are selectively labeled with different fluorescent proteins based on the expression of Cre recombinase.

BGHpA, a polyadenylation signal derived from bovine growth hormone gene; E/SYN, enhanced human synapsin I promoter (Hioki et al., 2007); ITR, inverted terminal repeat.