Figure 3.

Injection of the AAV vector solution

(A) PC-100 puller.

(B) Fabrication of a glass micropipette.

(C) Folding the tip of a glass micropipette

(D) Aspiration of the AAV vector solution into the glass micropipette. First, 500 nL of the solution is aspirated to mark the top surface with a permanent marker, and then another 200 nL is aspirated.

(E) Head fixation of an anesthetized mouse with a stereotaxic instrument.

(F) Perforation with a hand drill.

(G) Picospritzer III connected to a high-pressure N₂ cylinder.

(H) Attaching the glass micropipette to the holder of Picospritzer III.

(I) Inserting the micropipette into the brain.