Figure 6.

Signal enhancement with the BT-GO reaction

(A) Schematic diagram of the signal amplification method with the BT-GO reaction. BT is deposited on tyrosine residues in the tissue via the peroxidase activity of ABC. This deposition requires H₂O₂, and the chemical reaction between glucose and GO supplies H₂O₂ stably.

(B and C) Visualization of GFP-labeled dMSN axon fibers in the GPe without (B) or with (C) the BT-GO reaction. While DAB deposition through the peroxidase activity of ABC gave a weak signal (B), the BT-GO reaction markedly amplified the signals of the axon fibers. (C) is the adjacent section of (B). Scale bar, 100 μm.