Reagent | Final concentration | Amount |
---|---|---|
λ-carrageenan | 0.12% (w/v) | 120 mg |
Normal donkey serum | 1% (v/v) | 1 mL |
Triton X-100 | 0.3% (v/v) | 0.3 mL |
20% sodium azide solution | 0.02% (v/v) | 0.1 mL |
10× PBS | 1× | 10 mL |
ddH2O | N/A | up to 100 mL |
The solution may be preserved at 4°C for up to 1 year.