Reagent | Final concentration | Amount |
---|---|---|
Glycerol | 50% (v/v) | 10 mL |
DABCO | 2.5% (w/v) | 0.5 g |
20% sodium azide solution | 0.2% (w/v) | 20 μL |
10× PBS | 1× | 2 mL |
ddH2O | N/A | up to 20 mL |
The solution may be preserved at 4°C for up to 1 year.
Reagent | Final concentration | Amount |
---|---|---|
Glycerol | 50% (v/v) | 10 mL |
DABCO | 2.5% (w/v) | 0.5 g |
20% sodium azide solution | 0.2% (w/v) | 20 μL |
10× PBS | 1× | 2 mL |
ddH2O | N/A | up to 20 mL |
The solution may be preserved at 4°C for up to 1 year.