0.5 M Tris-HCl (pH 7.6)

Reagent	Final concentration	Amount
Tris	0.5 M	60.57 g
ddH2O	N/A	up to 1 L

Adjust pH to 7.6 with HCl.

Before use, dilute the solution 10-fold with ddH₂O (50 mM Tris-HCl).

The solution may be preserved at 20°C–25°C for up to 1 year.