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0.5 M Tris-HCl (pH 7.6)
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris | 0.5 M | 60.57 g |
| ddH2O | N/A | up to 1 L |
Adjust pH to 7.6 with HCl.
Before use, dilute the solution 10-fold with ddH2O (50 mM Tris-HCl).
The solution may be preserved at 20°C–25°C for up to 1 year.
