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. 2020 Dec 8;11:585203. doi: 10.3389/fgene.2020.585203

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Copyright © 2020 Rao, Mao, Xia, Zhang, Hu, Yuan, Yang, Yan, Deng, Cai, Mao, Li and Liao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MNGC formation and B. pseudomallei intracellular growth in A549 cells. (A) Confocal microscope shows the formation of MNGCs induced by B. pseudomallei at different infected times. Red is the B. pseudomallei indicated by Alexa Fluor 568, blue is the nucleus indicated by DAPI, and green is the cytoskeleton protein actin indicated by Actin-Tracker Green. (B) The percentage of MNGC formation was shown at 0, 6, and 12 hpi. (C) Intracellular growth of B. pseudomallei in A549 cell lines was assayed at different time points (1–20 h) following the initial 2 h infection with a MOI of 10. Three replicates were used for each time point. Bars represent the standard deviation of three replicates.