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. 2020 Jun 24;28:7–15. doi: 10.1016/j.jare.2020.06.006

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© 2020 The Authors. Published by Elsevier B.V. on behalf of Cairo University.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — LncRNA ZFAS1 facilitated the epigenetic silencing of HIF1A. (A) Lentiviral over-expression of LncRNA ZFAS1 was confirmed by RT-qPCR. (B) Western blot showed the protein level of HIF1 and EPAS1 after lentiviral over-expression of LncRNA ZFAS1. (C) ChIP analysis of H3K9me3, H3K27me3, and H3K4me3 on HIF1A promoter in GCA cells with or without overexpressing the LncRNA ZFAS1 via RT-qPCR. NC: negative control; GCA-H008: fresh isolated GCA cell line with high level of LncRNA ZFAS1; GCA-L084: fresh isolated GCA cell line with low level of LncRNA ZFAS1; *P < 0.05. The unpaired Student’s t test was used for two groups comparison.