Estrogen supplementation ameliorates pyroptosis and inflammation in cardiac aorta of OVX ApoE-/- mice and Hcy-treated HUVECs. (A) Representative western blots and relative quantitative analysis of NLRP3, cleaved caspase 1, and GSDMD in cardiac aorta of three groups of mice. (B) Levels of IL-1β in plasma of three groups of mice. (C) Levels of IL-18 in the plasma of three groups of mice. (D) Levels of Hcy in plasma of three groups of mice. (E) Representative western blots and relative quantitative analysis of NLRP3, cleaved caspase 1, and GSDMD in HVUECs cells. (F) Representative western blots and relative quantitative analysis of IL-1 and IL-18 in HVUECs cells. In all experiments, n = 6. **P < 0.01.