JUP expression and its location in gastric cancer tissues. (A) IHC staining to detect expression and location of JUP in different stages of gastric cancer tissues, such as carcinoma in situ (PT), lymph node metastasis tissues (N0-N3), distant metastasis tissues (M) (Scale bars, 100 µm). (B) Quantification of JUP expression was determined by IHC scores for carcinoma in situ (PT), lymph node metastasis tissues (N0-N3), distant metastasis tissues (M). (C) The ratio of nuclear JUP/cytoplasmic JUP in PT, N0-N3 and M. (D) The Kaplan-Meier survival analysis reveals lower expression of JUP to be related with poor prognosis.