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. 2020 Dec 21;40(12):BSR20201981. doi: 10.1042/BSR20201981

Figure 3. miR-26b-5p targeted COX2 in both human and rat species.

Figure 3

(A) The putative binding sites between miR-26b-5p and the 3′UTR of COX2. (B,C) qPCR was performed to test the expression of miR-26b-5p following 48 h of cell transfection with mimic-NC or mimic-miR-26b-5p. (D,E) The luciferase activity of COX2-WT was tested following 48 h of cell transfection with mimic-NC or mimic-miR-26b-5p. (F–I) qPCR and Western blotting assays were used to detect the mRNA and protein levels of COX2 following 48 h of cell transfection with mimic-NC or mimic-miR-26b-5p (n=3, *P<0.05, vs. mimic-NC group).