Skip to main content
. 2020 Dec 8;11:596103. doi: 10.3389/fimmu.2020.596103

Figure 2.

Figure 2

MARCO binds to unopsonized crystals. (A) Confocal microscopy of MSU (lot2, lot3), cholesterol, t-CPPD, silica, and S. cerevisiae. Particles were incubated at RT for 60 min with either Fc-hMARCO or Fc-mDectin-1 recombinant protein. After washing with HBSS, bound proteins were visualized using anti-human IgG AlexaFluor488 (anti-Fc AF488, green). Representative of at least two independent experiments; DIC = digital interference contrast; scale bar = 40 µm. (B) Indicated crystals were incubated in HBSS (unopsonized) or human serum from three individual healthy donors at 37°C for 30 min. After washing with HBSS the particles were incubated with 5 µg/ml recombinant His-tagged protein in HBSS + 5% BSA at 4°C for 60 min (hLDLR, hMARCO, hCD32b). Bound proteins were stained with anti-His Tag PE and the fluorescence of the particles was analyzed using a flow cytometer. Median fluorescent intensity (FI) and SEM are shown. Negative control = S. cerevisiae.