Figure 6.

IFN‐γ‐induced ACE2 expression is attenuated by glucocorticoids. A, Cultured primary nasal epithelial cells were incubated with IFN‐γ (10 ng/mL), IL‐4 (50 ng/mL), IL‐5 (50 ng/mL), IL‐13 (50 ng/mL) or IL‐17 (50 ng/mL) for 24 h, and then analysed for expression of ACE2 by RNA sequencing. N = 3 for IFN‐γ and IL‐13 group, n = 4 for IL‐4, IL‐5 and IL‐17 group. B, Cultured primary nasal epithelial cells (n = 3) were incubated with IFN‐γ (10 ng/mL) ± budesonide (0.4 μg/mL, 0.8 μg/mL) or dexamethasone (0.5 μg/mL, 1.0 μg/mL) for 24 h, and then analysed for expression of ACE2 by real‐time PCR. ACE2, angiotensin‐converting enzyme 2; IL, interleukin; FPKM, fragments per kilo‐base of exon per million fragments mapped; Bud, budesonide; Dex, dexamethasone