TABLE 1.

Main differences between 2D and 3D cell culture models

	2D cell culture	3D cell culture	References
Cell‐to‐cell contact	+	+++	32
Extracellular matrix production	+	+++	33
Higher cell density in vitro	++	+++	34
Production of pro angiogenic factors	+	++	35
Capacity of large‐scale tissue production	+	++	36
Use for high‐throughput systems and drug screening tests	++	+++	37
Mimicry in vivo tissue microenvironments	+	+++	38
Mimicry of embryogenesis and organogenesis processes	+	+++	39