FIGURE 4.

The Clmp ^+/− mice show excessive accumulation of the inflammatory factors in the infarcted myocardium. A, Through RNA‐Seq analysis, gene expression changes in the MI LV were showed with Volcano Plot by pair comparison (Clmp ^+/− vs wild‐type, n = 3). Red and blue dots indicate significantly differentially expressed genes. B, The heatmap depicted 52 differentially expressed genes at week 2 after MI between the Clmp ^+/− and wild‐type mouse hearts. C, The GO term enrichment analysis indicated that the changed genes were mainly involved in the secreted and inflammatory response. D‐K, The qRT‐PCR assays showed the expression levels of Clmp and the inflammatory‐associated genes IL‐1β, C4b, Ccl8, Igkc, Ighm, Ighg1 and J chain in the wild‐type sham, wild‐type MI and Clmp ^+/− MI LVs. Data are presented as the mean ± SEM. Student's t test or one‐way ANOVA; *P < .05; **P < .01