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. 2020 Oct 21;24(23):13775–13788. doi: 10.1111/jcmm.15955

FIGURE 5.

FIGURE 5

Clmp knockdown results in fibroblast pyroptosis and inflammation. A, The representative Western blot imaging of CLMP in the wild‐type sham, wild‐type MI and Clmp +/− MI LVs on the indicated days post‐MI. B, The relative densitometric quantification of CLMP protein on day 3 post‐MI in panel E (n = 3). C, The representative Western blot imaging of CASP1 in the wild‐type sham, wild‐type MI and Clmp +/− MI hearts on the indicated days post‐MI. D,The relative densitometric quantification of active CASP1 (P20) protein on day 3 post‐MI in panel A (n = 3). E,The representative Western blot imaging of GSDMD in the wild‐type sham, wild‐type MI and Clmp +/− MI LVs on the indicated days post‐MI. GSDMD‐FL, GSDMD full‐length; GSDMD‐N, GSDMD cleavage. F, The relative densitometric quantification of GSDMD‐N protein on day 3 post‐MI in panel C (n = 3). G, IL‐1β expression in the wild‐type MI and Clmp +/− MI mice from ELISA. H, Cleaved IL‐1β protein by Western blot analysis. I, The serum LDH concentrations in the wild‐type MI and Clmp +/− MI mice on day 3 post‐MI (wild‐type: n = 5; Clmp +/−: n = 4). J, The myeloperoxidase analysis in wild‐type MI and Clmp +/− MI LV (wild‐type: n = 5; Clmp +/−: n = 4). K, The qRT‐PCR analysis of Ly6g among the wild‐type sham, wild‐type MI and Clmp +/− MI LV (wild‐type: n = 3; Clmp +/−: n = 3. L‐N, The qRT‐PCR analysis of Clmp, Casp1 and Gsdmd in the adult cardiac fibroblasts (CFs) and cardiomyocytes (CMs). O, The representative images of immunostaining for CASP1, GSDMD and TNNT2 in the ischaemic hearts. Hoechst 33342 was used to label nuclei. Scale bar indicates 100 μm. P, The representative Western blot imaging of CASP1 in isolated adult wild‐type and Clmp +/− CFs with pyroptosis induction. Q, The relative densitometric quantification of panel L. R, The representative Western blot imaging of GSDMD in the isolated adult wild‐type and Clmp +/− CFs with pyroptosis induction. S, The relative densitometric quantification of panel N. T, The levels of LDH in the supernatant of isolated adult wild‐type and Clmp +/− fibroblasts after pyroptosis induction (wild‐type: n = 5; Clmp +/−: n = 4). All data are presented as the mean ± SEM. Student's t test or one‐way ANOVA; *P < .05; **P < .01