Figure 1.

Overexpression of miR‐214 and brain injury are observed in hippocampus of rats after general anaesthesia. A, The expression of miR‐214 in brain tissues of normal rats and rats exposed to 2% sevoflurane determined by RT‐qPCR. B, The rat brain tissue injury in normal rats and rats exposed to 2% sevoflurane detected by HE staining (× 200). C, The expression of pro‐inflammatory cytokines (IL‐6 and TNF‐α) in the peripheral blood of normal rats and rats exposed to 2% sevoflurane determined by ELISA. D, Measurement of neuronal apoptosis in normal and rats exposed to 2% sevoflurane detected by TUNEL assay (×400). E, The contents of SOD, GSH and MDA in brain tissues of normal rats and rats exposed to 2% sevoflurane. F, Mitochondrial oxidative respiratory activity in brain tissues of normal rats and rats exposed to 2% sevoflurane. G, The protein expression of Bdnf and Creb, as well as the extent of Creb phosphorylation normalized to GAPDH in brain tissues of normal rats and rats exposed to 2% sevoflurane determined by Western blot analysis. *P < .05 vs normal rats. The data were measurement data and expressed as mean ± standard deviation. The data between two groups obeying normal distribution and homogeneous variance in unpaired design were compared using unpaired t test (n = 12)