Figure 2.

General anaesthesia impairs Mfn2 interaction with Pkm2 in rats. A, The expression of Mfn2 and Pkm2 in normal rats and rats exposed to 2% sevoflurane detected by immunohistochemistry (×400). *P < .05 vs the normal rats. B, The expression of Mfn2 and Pkm2 normalized to GAPDH in normal rats and rats exposed to 2% sevoflurane determined by Western blot analysis. *P < .05 vs the normal rats. C, The interaction between Mfn2 and Pkm2 verified by Co‐IP. *P < .05 vs IgG. D, The co‐localization of Mfn2 and Pkm2 detected using fluorescent double‐labelling technique (×400). *P < .05 vs the normal rats. The data were measurement data and expressed as mean ± standard deviation. The data between two groups obeying normal distribution and homogeneous variance in unpaired design were compared using unpaired t test (n = 12)