Figure 5.

Adenovirus infection of miR‐214 inhibitor ameliorates brain injury in rats following general anaesthesia. A, The expression of miR‐214 in brain tissues of rats undergoing different treatments determined by RT‐qPCR. B, The expression of Mfn2 in brain tissues of rats undergoing different treatments detected by immunohistochemistry (×400). C, The expression of pro‐inflammatory cytokines (IL‐6 and TNF‐α) in the peripheral blood of rats undergoing different treatments determined by ELISA. D, Measurement of neuronal apoptosis in rats undergoing different treatments detected by TUNEL assay (×400). E, The content of SOD, GSH and MDA in brain tissues of rats undergoing different treatments. F, The protein expression of Bdnf, Creb and the extent of Creb phosphorylation normalized to GPADH in brain tissues of rats undergoing different treatments determined by Western blot analysis. *P < .05 vs NC inhibitor. The data were measurement data and expressed as mean ± standard deviation. The data between two groups obeying normal distribution and homogeneous variance in unpaired design were compared using unpaired t test (n = 12)