FIGURE 4.

Activation of DNA methyltransferases was induced by IL‐1β via iNOS. INS‐1 cells were treated with 1 ng/mL IL‐1β for 48 h. For DNMT3a, DNMT3b and DNMT1, the mRNA levels and protein levels were analysed by real‐time PCR (A) and Western blot analysis (B), respectively. β‐Tubulin was used as the internal control. (C) The mRNA levels of these DNMT were detected in rat islets treated with 1 ng/mL IL‐1β for 48 h. (D) The expression level of iNOS, Akt, p‐Akt was detected by Western blot analysis. Data are presented as the mean ± SEM (n = 3) of three independent experiments. *P < .05, **P < .01