FIGURE 4.

MCM8 knockdown inhibits cell growth. A, MCM8 mRNA levels in AGS and HGC27 cells were detected by RT‐qPCR. ***P < .001, ****P < .0001. B, Analysis of Western blotting showing that protein levels of MCM8 are reduced after MCM8 knockdown. C, Semi‐quantification of Western blotting. The integrated band density was determined using the ImageLab Software, and β‐actin was used as the reference. *P < .05. D, MTT assay showing that MCM8 knockdown inhibits cell viability. ns: not significant. *P < .05. E, Morphological changes in AGS and HGC cells after infection with sh‐MCM8 vs sh‐Ctrl lentiviruses for 72 h. Cell morphology was observed under a phase‐contrast microscope. Images were obtained at a magnification of 200×. F, MCM8 knockdown inhibits cell growth. *P < .05, **P < .01. G, Representative images of colony formation assay. H, MCM8 knockdown reduces the number of colonies. *P < .05, **P < .01. I, Representative images of cell cycle assay by flow cytometry. J, MCM8 knockdown induces arrest at the G2/M phase. **P < .01. RT‐qPCR, quantitative reverse transcription polymerase chain reaction