FIGURE 5.

MCM8 knockdown induces cell apoptosis. A, Detection of apoptotic cells stained with Annexin V‐FITC and 7‐AAD by flow cytometry. B, MCM8 knockdown promotes cell apoptosis. **P < .01. C, Determination of Bcl‐2 and Bax mRNA levels using RT‐qPCR. GAPDH was used as a reference. *P < .05, ***P < .001, ***P < .0001. D, Protein levels of Bcl‐2, Bax and cleaved caspase‐3 were determined using Western blotting. β‐actin was used as the loading control. E, Semi‐quantification of Western blotting. The integrated band density was determined using ImageLab Software, and β‐actin was used as the reference. **P < .01, ***P < .001. RT‐qPCR, quantitative reverse transcription polymerase chain reaction