Figure 4.

Tris DBA reduced the MAPK (ERK, JNK)‐mediated priming signal of the NLRP3 inflammasome in J774A.1 macrophages. A, IL‐1β secretion by J774A.1 macrophages measured by ELISA; B, IL‐1β and (C) caspase‐1 protein levels in J774A.1 macrophages assessed by Western blot analysis. J774A.1 macrophages incubated for 1 h with Tris DBA, then for 5.5 h with or without IgA‐ICs, and then for 30 min with or without 5 mmol/L ATP; D, NLRP3 and pro‐IL‐1β protein levels measured by Western blot and semiquantitative analysis. Cells incubated for 1 h with Tris DBA, then for 5.5 h with or without IgA‐ICs; E, JNK, ERK and p38 MAPK signalling pathways phosphorylation levels in J774A.1 macrophages measured by Western blot analysis and semiquantitative analysis; NLRP3 and pro‐IL‐1β protein levels measured by Western blot analysis. Cells incubated for 1 h with (F) PD98059 or (G) SP600125 then for 5.5 h with or without IgA‐ICs. PD98059, an ERK inhibitor, SP600125, a JNK inhibitor. The data are expressed as the means ± SEM for three separate experiments. ns, no difference, *P < .05, **P < .01, ***P < .005, ****P < .001