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. 2020 Dec 19;6(12):e05734. doi: 10.1016/j.heliyon.2020.e05734

Figure 1.

Figure 1

miR-1911-3p is an effector for mEAK-7 and mTOR signaling in human lung cancer cells. H1975 (A) and H1299 (B) cells were transfected with miR-control or miR-1911-3p mimic for 48 h to evaluate mEAK-7 and mTOR signaling. GAPDH was used as a loading control. See full images in Supplementary Figure S1. Expression of miR-1911-3p (C) and MEAK7 (D) in H1299, H1975, and BEAS-2B cells was quantified by qPCR. microRNA and mRNA levels were normalized to RNU6-1 and GAPDH, respectively. Data are expressed as means ± SD of 3 independent experiments. n.s., not significant. (E) Expression of mEAK-7 and mTOR signaling in H1299, H1975, and BEAS-2B cells was analyzed by Western blotting. β-actin was used as a loading control. See full images in Supplementary Figure S2. All experiments were repeated at least three times.