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. 2020 Dec 19;6(12):e05734. doi: 10.1016/j.heliyon.2020.e05734

Figure 4.

Figure 4

miR-1911-3p targets MEAK7 mRNA at 3′-UTR. H1975 (A) and H1299 (B) cells were transiently co-transfected with 100 nM of miR-control or miR-1911-3p mimic in combination with 0.5μg of the empty luciferase reporter (pmirGLO) or pmirGLO-3′-UTR MEAK7. Luciferase activities were measured and normalized with basal Renilla luciferase activities. H1975 (C) and H1299 (D) cells were transiently co-transfected with 100 nM of miR-control or miR-1911-3p inhibitor in combination with 0.5μg of the empty luciferase reporter (pmirGLO) or pmirGLO-3′-UTR MEAK7. Luciferase activities were measured and normalized with basal Renilla luciferase activities. (E) Predicted consequential pairing of MEAK7 3′-UTR and miR-1911-3p. Pairing was performed using TargetScan (www.targetscan.org). All experiments were repeated at least three times. Data are expressed as means ± SE of 3 independent experiments (∗P < 0.05). n.s., not significant.