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. 2020 Dec 19;6(12):e05734. doi: 10.1016/j.heliyon.2020.e05734

Figure 5.

Figure 5

miR-1911-3p impairs co-localization of mTOR to the lysosome. (A) H1299 cells were treated with miRNA control or miR-1911-3p for 48 h. 250,000 cells were seeded onto glass well slides and allow to settle for 24 h. Cells were then starved for 1 h, refed with 10% FBS for 1 h, and processed for immunofluorescence analysis against mTOR and LAMP2 (lysosomal marker). White arrowheads indicate colocalization of mTOR and LAMP2. Scale bars, 100 μm. (B) Quantitative analysis of colocalization of mTOR and LAMP2 for (A). Six confocal images per condition were used for quantification.