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IFI16 knockdown in primary CD4+ T lymphocytes
Human CD4+ T lymphocytes isolated from three different donors were transfected with either a control or an IFI16-specific shRNA. Knockout efficiency was assessed by western blot. The right panel shows the quantification of IFI16 and PYHIN1 band intensities in the presence of the non-targeting (nt) control or an IFI16-specific shRNA. Data are represented as means ± SD.
