REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
IRDye 680RD goat anti-mouse IgG (H + L), 1:20,000	LI-COR	Cat# 926-68070 RRID:AB_10956588
IRDye 800CW goat anti-mouse IgG (H + L), 1:20,000	LI-COR	Cat# 926-32210 RRID:AB_621842
IRDye 680RD goat anti-rat IgG (H + L), 1:20,000	LI-COR	Cat# 926-68076 RRID: AB_10956590
IRDye 800CW goat anti-rat IgG (H + L), 1:20,000	LI-COR	Cat# 926-32219, RRID: AB_1850025
Mouse monoclonal anti-IFI16, 1:150	Santa Cruz	Cat# sc-8023 RRID: AB_627775
Rat monoclonal anti-GAPDH, 1:1,000	BioLegend	Cat# 607902 RRID: AB_2734503
Rabbit monoclonal anti-actin, 1:5,000	Abcam	Cat# ab8226; RRID: AB_306371
Bacterial and virus strains
XL2-Blue MRF′ ultracompetent cells	Agilent Technologies	Cat# 200151
Biological samples
Human: peripheral blood mononuclear cells	DRK-Blutspendedienst Baden-Württemberg-Hessen, Ulm, Germany	N/A
Oligonucleotides
Non-targeting (NT) gRNA: ACGGAGGCTAAGCGTCGCAA	IDT	N/A
IFI16 gRNA: GACCAGCCCTATCAAGAAAG	IDT	N/A
CTRL siRNA: UUCUCCGAACGUGUCACGUdTdT	Eurofins	N/A
IFI16 siRNA (1); GGUGCUGAACGCAACAGAAUCAUUU AAAUGAUUCUGUUGCGUUCAGCACC		Cat# IFI16HSS105205
IFI16 siRNA (2): GGAUUGAUUAGAAGUGCCAGCGUAA UUACGCUGGCACUUCUAAUCAAUCC	Invitrogen	Cat# IFI16HSS105206
IFI16 siRNA (3): UUAGUUUGUGAGCAAAGCUGAUUGA UCAAUCAGCUUUGCUCACAAACUAA	Invitrogen	Cat# IFI16HSS105207
IFI16 shRNA	Origine	Cat# TR312254
Chemicals and reagents
Amaxa 4D-Nucleofector Human Activated T cell P3 Lonza Kit	Lonza	Cat# V4XP-3032
Phosphate-buffer saline (PBS)	Thermo Fisher	Cat#14190144
ACK lysis buffer (1×)	Lonza	Cat# 10-548E
HiFi Cas9 nuclease V3	IDT	Cat# 1081061
L-Glutamine	Pan Biotech	Cat# P04-80100
Dulbecco’s modified Eagle’s medium (DMEM)	Gibco	Cat# 41965039
Opti-MEM	Gibco	Cat# M3942
Roswell Park Memorial Institute (RPMI)	Gibco	Cat# 22400089
Penicillin-streptomycin	Thermo Fisher	Cat# 15140122
FCS	Gibco	Cat# 10270106
β-Mercaptoethanol	Sigma-Aldrich	Cat# 444203
Recombinant human IL-2	MACS Miltenyi Biotec	Cat# 170076147
Lipofectamine RNAiMAX	Thermo Fisher	Cat# 13778075
Anti-CD3/CD28 magnetic beads	Life Technologies	Cat# 11131D
Biocoll separating solution	Biochrom	Cat# L6113
RosetteSep human CD4+ T cell enrichment cocktail	STEMCELL Technologies	Cat# 15022
4× protein sample loading buffer	LI-COR	Cat# 928-40004
Complete ULTRA inhibitor cocktail tablet	Roche	Cat# 05892791001
Human M-CSF	R&D Systems	Cat# 216-MC-01M
Plasmid midi kit	QIAGEN	Cat# 12145
SOC medium	Invitrogen/Life Technologies	Cat# 15544034
Software and algorithms
Corel DRAW 2017	Corel Corporation	https://www.coreldraw.com/
GraphPad Prism version 8	GraphPad Software	https://www.graphpad.com RRID: SCR_002798
LI-COR Image Studio Lite version 5.0	LI-COR	www.licor.com/ RRID: SCR_013715