. Author manuscript; available in PMC: 2020 Dec 22.

Published in final edited form as: Food Chem Toxicol. 2020 Jan 3;136:111114. doi: 10.1016/j.fct.2020.111114

Table 1.

Spleen phenotyping following dermal exposure to PFOA.

Exposure Duration	4 Days				14 Days

PFOA (w/v)	0%	0.5%	1%	2%	0%	0.5%	1%	2%
Cellularity (× 10⁷)	9.48 ± 1.29	6.52 ± 0.40^**	4.54 ± 0.38^**	4.59 ± 0.47^**	8.85 ± 0.75	3.78 ± 0.62^**	0.86 ± 0.10^**	0.82 ± 0.08^**
CD4⁺ (× 10⁷)	2.24 ± 1.24	1.67 ± 1.38^**	1.11 ± 0.85^**	1.19 ± 0.13^**	1.93 ± 0.17	1.14 ± 0.15^**	0.30 ± 0.02^**	0.27 ± 0.02^**
CD4⁺ (%)	23.70 ± 0.43	25.50 ± 0.53	24.72 ± 0.48	25.90 ± 1.22	21.82 ± 0.74	31.02 ± 1.69^**	36.50 ± 1.95^**	33.58 ± 1.00^**
CD8⁺ (× 10⁶)	10.85 ± 0.57	7.62 ± 0.45^**	5.07 ± 0.44^**	5.32 ± 0.51^**	9.19 ± 0.80	5.22 ± 0.78^**	1.33 ± 0.11^**	1.28 ± 0.12^**
CD8⁺ (%)	11.48 ± 0.19	11.70 ± 0.28	11.16 ± 0.32	11.64 ± 0.34	10.41 ± 0.39	14.00 ± 0.42^**	15.84 ± 0.76^**	15.62 ± 0.57^**
B-cells (× 10⁷)	3.50 ± 0.22	2.45 ± 0.14^**	1.69 ± 0.16^**	1.74 ± 0.19^**	3.45 ± 0.33	1.32 ± 0.24^**	0.27 ± 0.03^**	0.21 ± 0.03^**
B-cells (%)	36.94 ± 0.70	37.68 ± 0.73	37.14 ± 0.47	37.84 ± 0.51	38.82 ± 0.61	34.42 ± 1.22	25.72 ± 1.68^**	25.20 ± 1.73^**
Dendritic Cells (× 10⁵)	9.98 ± 0.11	7.12 ± 0.15^**	5.30 ± 0.54^**	5.63 ± 0.54^**	7.80 ± 0.68	4.32 ± 0.64^**	0.76 ± 0.12^**	0.88 ± 0.10^**
Dendritic Cells (%)	1.06 ± 0.05	1.10 ± 0.05	1.16 ± 0.07	1.23 ± 0.04	0.88 ± 0.03	1.16 ± 0.06^**	0.86 ± 0.07	1.06 ± 0.04
Monocytes (× 10⁶)	3.73 ± 0.31	2.53 ± 0.78^**	1.75 ± 0.17^**	1.72 ± 0.18^**	3.31 ± 0.35^**	1.15 ± 0.18^**	0.42 ± 0.07^**	0.50 ± 0.03^**
Monocytes (%)	3.92 ± 0.16	3.93 ± 0.20	3.84 ± 0.14	3.78 ± 0.23	3.71 ± 0.16	3.10 ± 0.18	4.97 ± 0.56	6.35 ± 0.62^**

Spleen immune phenotyping following 4 and 14 days of PFOA exposure. Numbers represent mean (± SE) of 5 mice per group. Levels of statistical significance are denoted (**p < 0.01 and * p < 0.05) as compared to acetone vehicle (0%).