. Author manuscript; available in PMC: 2020 Dec 22.

Published in final edited form as: Food Chem Toxicol. 2020 Jan 3;136:111114. doi: 10.1016/j.fct.2020.111114

Table 2.

Lymph node phenotyping following dermal exposure to PFOA.

Exposure Duration	4 Days				14 Days

PFOA (w/v)	0%	0.5%	1%	2%	0%	0.5%	1%	2%
Cellularity (× 10⁶)	4.50 ± 0.85	5.11 ± 0.66	4.94 ± 0.53	9.02 ± 1.31^*	4.69 ± 0.65	3.73 ± 0.29	2.34 ± 0.23^*	2.86 ± 0.42^**
CD4⁺ (× 10⁶)	2.34 ± 0.43	2.70 ± 0.35	2.60 ± 0.26	4.70 ± 0.68^*	2.57 ± 0.35	2.06 ± 0.19	1.09 ± 0.13^**	1.20 ± 0.19^**
CD4⁺ (%)	52.12 ± 0.93	52.94 ± 0.50	52.96 ± 0.65	52.30 ± 1.06	54.82 ± 0.96	55.10 ± 0.78	46.18 ± 1.55^*	41.90 ± 1.73^**
CD8⁺ (× 10⁶)	1.09 ± 0.16	1.27 ± 0.16	1.18 ± 0.13	2.19 ± 0.31^*	1.11 ± 0.14	1.01 ± 0.61	0.73 ± 0.07	0.87 ± 0.01
CD8⁺ (%)	24.90 ± 1.07	24.98 ± 0.28	23.84 ± 0.53	24.36 ± 0.23	23.78 ± 0.63	27.36 ± 0.91	31.53 ± 1.39^**	30.76 ± 0.30^**
B-cells (× 10⁶)	0.72 ± 0.17	0.82 ± 0.11	0.85 ± 0.10	1.44 ± 0.23^*	0.87 ± 0.14	0.53 ± 0.04	0.39 ± 0.01^*	0.57 ± 0.10
B-cells (%)	15.42 ± 1.23	16.00 ± 0.78	17.18 ± 0.85	15.92 ± 0.96	18.42 ± 1.17	14.32 ± 0.58^*	17.28 ± 1.41	19.64 ± 1.17
Dendritic Cells (× 10⁴)	3.02 ± 0.82	3.43 ± 0.44	3.39 ± 0.41	9.24 ± 0.13^**	2.36 ± 0.27	2.04 ± 0.19	1.63 ± 0.29	2.14 ± 0.23
Dendritic Cells (%)	0.63 ± 0.08	0.67 ± 0.04	0.69 ± 0.04	1.03 ± 0.08^**	0.51 ± 0.03	0.55 ± 0.05	0.68 ± 0.06	0.77 ± 0.07^**
Monocytes (× 10⁵)	2.25 ± 0.44	1.82 ± 0.18	1.66 ± 0.19	4.06 ± 0.71^*	1.78 ± 0.28	1.36 ± 0.15	1.03 ± 0.16	1.41 ± 0.27
Monocytes (%)	5.20 ± 0.91	3.68 ± 0.29	3.35 ± 0.08^*	4.43 ± 0.29	3.80 ± 0.38	3.71 ± 0.46	4.34 ± 0.47	4.87 ± 0.54^**

Lymph node immune phenotyping following 4 and 14 days of PFOA exposure. Numbers represent mean (± SE) of 5 mice per group. Levels of statistical significance are denoted (**p < 0.01 and * p < 0.05) as compared to acetone vehicle (0%).