Figure 2.

PAK1 deficiency can promote regulatory T cells and attenuate inflammation response in vivo. (A, B) Flow cytometry analysed the expression of CD4⁺ T cells in the livers from WT and PAK1^−/− mice with or without S japonicum infection. (C, D) Flow cytometry analysed the CD4⁺CD25⁺ Foxp3⁺ (Treg) cell population in the hepatic lymphocytes. (E–G) The mRNA levels of IL‐4, IL‐10 and IL‐13 in the livers from WT and S japonicum‐infected mice were analysed by real‐time quantitative PCR (n = 10). (H, I) Flow cytometry analysed the proportion of CD4⁺CD25⁺ Foxp3⁺ (Treg) in the spleen. Data were representative one experiment from two replicate experiments each with n = 5 mice per group. Data were presented as mean ± SEM. *P < .05; **P < .005; ***P < .001