Figure 3.
AMPK mediates the inhibitory effect of metformin on alkaline phosphatase activity. A&B. MC3T3‐E1 cells were infected with varying multiplicity of infection (MOI) of adenovirus expressing a constitutively active mutant of AMPK α 1 (Ad‐AMPK‐CA) (A), or the dominant negative mutant (AMPK‐DN) (B) for 48 hours and then were incubated with osteogenesis differentiation media in the presence or absence of 1mM metformin (Met). GFP adenovirus was used as a control. Alkaline phosphatase activity was measured one week after the induction. C&D. The graph represents averages of 3 independent experiments from A and B, respectively (mean ± SD, N = 3). Statistical analysis was performed using Student's t test, and p values between groups were indicated (*P < .05). NM, no differentiation media, α‐MEM complete medium