Figure 4.

The effect of metformin on mineralization. MC3T3‐E1 cells were incubated with osteogenesis differentiation media in the presence or absence of varying doses of metformin for 21 days and stained with alizarin red. A. Representative images of differentiated cells. B. Graph represents alizarin deposit quantitation from 3 independent experiments (mean ± SD, N = 3). *Significant difference from metformin versus control was assessed by Student's t test (*P < .05). NM: no differentiation media, α‐MEM complete medium