Figure 5.

Metformin prevents trauma‐induced HO in mice. A. Mice received Achilles tenotomy on the right hindlimb and burn injury on the dorsum. The next day, they were injectedi.p.with vehicle (PBS) or metformin every 3 days for 8 weeks (vehicle, n = 6, metformin, n = 5). The injured limbs were examined by microCT at 8 weeks post‐injury. Representative 3D microCT reconstructions and serial 2D cross‐sections are shown in Figure A. Scale bar = 1mM, white arrows indicate ectopic bone. (B). The volume of HO was quantified from each group. * Statistical analysis was performed using Student's t test, and p values between metformin‐treated and vehicle‐treated group were indicated (*P < .05; vehicle, n = 6; metformin, n = 5)