Figure 6.
Effect of metformin on histological and molecular changes in the trauma‐induced HO. A. Representative H&E staining of HO at 8 wk after trauma. The injured tissues collected from vehicle‐treated control and metformin‐treated mice were sectioned and examined by H&E (vehicle, n = 6; metformin, n = 5). Areas of interest in sections were examined at different magnification, and the scale bar is indicated. B. Effect of metformin on the expression of Smad6, ALK2 and Smurf1 in injured tissue. Western blot was performed with antibodies, as indicated. C. Graphs represent scan densitometric ratio of bands from B. Statistical analysis was performed using Student's t test, and p values between metformin‐treated and vehicle‐treated group were indicated, *P < .05, (vehicle, n = 6; metformin, n = 5)