FIGURE 2.

Down‐regulated circFAT1 inhibits HCC cell proliferation, invasion and EMT level. (A) Hep3B and Huh7 cells were, respectively, transfected with sh‐control and sh‐circFAT1. The transfection efficiencies were analysed by qRT‐PCR. (B) Determination of shRNA‐treated Hep3B and Huh7 cells proliferation by performing CCK‐8 assays. (C) Cell invasion was detected by using transwell invasion assays. Invasion cells were amounted and shown. (D) The expression of EMT protein biomarkers E‐cadherin, N‐cadherin and vimentin in shRNA‐treated Hep3B and Huh7 cells was measured by Western blot; the expression of EMT proteins was analysed. All experiments were repeated at least three times.**P < .01,***P < .001