FIGURE 3.

CircFAT1 acts as miR‐30a‐5p sponge. (A) RIP experiments with AGO2 antibody were conducted, and qRT‐PCR assays were used to detect the expression of circFAT1 and miR‐30a‐5p. (B) qRT‐PCR assays with specific circFAT1 probe were performed to measure the expression of potential sponging RNAs (Starbase 3.0). (C) The putative binding sites between circFAT1 WT/ MuT and miR‐30a‐5p. (D and E) Luciferase activity was detected in luciferase reporter vectors harbouring circFAT1 WT/MuT sequences and miR‐30a‐5p mimics co‐transfected Huh7 and 293T cells. (F) Determination of the miR‐30a‐5p in sh‐NC or sh‐circFAT1 transfected Huh7 and Hep3B cells. All experiments were repeated at least three times. **P < .01