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. 2020 Nov 11;24(24):14561–14570. doi: 10.1111/jcmm.16085

FIGURE 4.

FIGURE 4

MiR‐30a‐5p directly interacts with REEP3. (A) Determination of selected mRNAs expression by qRT‐PCR in Huh7 cells transfected with inhibitor control or miR‐30a‐5p inhibitor, respectively. (B) Measurement of selected mRNAs expression using qRT‐PCR in Huh7 cells transfected with sh‐control or sh‐circFAT1, respectively. (C) The putative binding sites between REEP3 wild‐type (WT) or mutant type (MuT) and miR‐30a‐5p. (D and E) Luciferase activity was detected in luciferase reporter vectors harbouring REEP3 WT or Mut sequences and miR‐30a‐5p mimics co‐transfected Huh7 and 293T cells. (F) Huh7 cells were pre‐transfected with mimic controls, miR‐30‐5p mimics, miR‐30‐5p mimics + OE‐NC and miR‐30‐5p mimics + OE‐circFAT1, respectively. The expression of REEP3 in treated Huh7 cells was detected by Western blot. (G) Huh7 cells were pre‐transfected with OE‐NC, OE‐circFAT1, NC‐mimic + OE‐circFAT1 and miR‐30‐5p mimic + OE‐circFAT1, respectively. The expression of REEP3 in treated Huh7 cells was detected by Western blot. Comparative statistics were shown. All experiments were repeated at least three times. *P < .05, **P < .01, ***P < .001