FIGURE 5.

CircFAT1 promotes HCC development through miR‐30a‐5p/REEP3 pathway. HCC cell lines Hep3B and Huh7 were pre‐transfected with sh‐control, sh‐circFAT1, sh‐circFAT1 + OE‐NC and sh‐circFAT1 + OE‐REEP3, respectively. (A) The expression of REEP3 was detected using Western blot. (B) To detect cells proliferation ability, treated Hep3B and Huh7 cells were subjected to CCK‐8 assays. (C‐D) Transwell invasion assays were conducted to detect cells invasion ability, invasion cells were counted, and comparative statistics were shown. (E) The EMT biomarkers E‐cadherin, N‐cadherin and Vimentin level in treated cells were measured by ELISA assays. All experiments were repeated at least three times. *P < .05, **P < .01