FIGURE 5.

CDK16 was a direct target of miR‐584‐5p. (A) A Venn drawing showed the overlapping of the target genes of miR‐584‐5p predicted by GSE101728, miRDB, TargetScan and starbase. (B) The expression of CDK16 in HCC tissues (n = 7) and adjacent normal tissues (n = 7) in GSE108724. **P < 0.01 compared to adjacent normal tissues. (C) The binding site between miR‐584‐5p and CDK16 3’‐UTR. (D) Dual‐luciferase reporter assay showed that miR mimics significantly reduced luciferase activity of WT‐CDK16 group. **P < 0.01 compared to miR‐NC group. (E) The protein expression of CDK16 in the human normal liver cell line (L02) and HCC cell lines (SK‐HEP‐1, Hep G2, Huh‐7 and Hep 3B) were determined by Western blot. **P < 0.01 compared to L02. (F) The protein expression of CDK16 in Huh‐7 and Hep 3B cells after si‐1306, miR‐inhibitor, OV‐CDK16 or co‐transfection was tested by qPCR. **P < 0.01 compared to control group. ##P < 0.01 compared to si‐1302 group. ΔΔP < 0.01 compared to OV‐CDK16 group. All data represent the mean ± SD