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. 2020 Nov 9;24(24):14549–14560. doi: 10.1111/jcmm.16083

Figure 4.

Figure 4

Farnesoid X receptor (FXR) activation suppresses JAK2/STAT3 pathway by up‐regulating SOCS3 expression in colon cancer cells. A, Western blot bands of the critical proteins of JAK/STAT pathway in Caco‐2 and HT‐29 cells after obeticholic acid (OCA) exposure. B, Quantitative analysis of the critical proteins of JAK/STAT pathway in Caco‐2 and HT‐29 cells. C, Real‐time PCR of SOCS3 levels in Caco‐2 and HT‐29 cells after OCA exposure. D, Western blot bands of the critical proteins of JAK/STAT pathway in OCA‐treated Caco‐2 and HT‐29 cells upon SOCS3 knockdown. E, Quantitative analysis of the critical proteins of JAK/STAT pathway in Caco‐2 and HT‐29 cells. F and G, Immunofluorescence staining of STAT3 in Caco‐2 (F) and HT‐29 (G) after OCA exposure. *P < .05