Figure 3.

pSTAT3 transcriptionally suppresses miR‐19b/1281 to up‐regulate NFAT5 expression. A, a heatmap for top 20 differentially expressed miRNAs in mouse lung tissues following AG‐490 treatment determined by a miRNA transcriptome analysis; B, miR‐126, miR‐19b, miR‐392, miR‐1281 and miR‐378 expression in lung tissues evaluated using RT‐qPCR; C, binding sites between pSTAT3 and the promoter sequences of miR‐19b, miR‐1281 and miR‐378 predicted on JASPAR System; D, conservative binding sequence of pSTAT3; E, binding relationship between pSTAT3 and miR‐19b, miR‐1281 or miR‐378 validated by ChIP‐qPCR; F, shared downstream target mRNAs of miR‐19b and miR‐1281 predicted on StarBase and TargetScan; G, binding relationship between miR‐19b/1281 and NFAT5 validated by dual‐luciferase reporter gene assays; H‐I, NFAT5 expression in mouse lung tissues determined by RT‐qPCR (H) and Western blot analysis (I), respectively. Three independent experiments were performed. N = 8 in each group. Data were expressed as mean ± SD. In panels H and I, data were analysed using one‐way ANOVA, while data in panels B, E and G were determined by two‐way ANOVA, and Tukey's multiple comparison for post hoc test after ANOVA. *P < .05, **P < .01