Figure 4.

miR‐19b/1281 agomiR or sh‐NFAT5 reduces inflammation in lung tissues and lung epithelial cells in T2DM‐PTB mice. A, 1 mo after diabetes induction, mice were exposed to ~100 CFU of aerosolized Mtb; another 30 d later, each mouse was further given miR‐19b/1281 agomiR, or the shRNA of NFAT5 (5 nmol/100 μL) twice every 10 d through i.p for a total of 30 d; B, expression of miR‐19b, miR‐1281 and NFAT5 mRNA in lung tissue homogenate determined by RT‐qPCR; C, protein levels of TNF‐α, IL‐6 and IL‐1β in lung tissues determined by ELISA kits; D, survival time of mice after Mtb infection (n = 10); E, Mtb content in mouse lung tissues; F, lung fibrosis detected using Masson's trichrome staining; G, apoptosis in lung tissues determined by dUTP nick end labelling (TUNEL). Each spot in the images indicates one sample. N = 8 in each group. Three independent experiments were performed. Data were expressed as mean ± SD. In panel B, data were analysed using the unpaired t test, while data in panels C, E, F and G were analysed by one‐way ANOVA and Tukey's multiple comparison test. *P < .05, **P < .01