Figure 7.

Overexpression of NFAT5 blocks the role of AG‐490 in mice. A, 1 mo after ~100 CFU of aerosolized Mtb infection, mice were given with AG‐490 (1 mg/kg) through i.p twice every 10 d through i.p for a total of 30 d, another 30 d later, each mouse was further given LV overexpressing NFAT5 (5 nmol/100 μL); B, protein level of NFAT5 in mouse lung tissues determined by Western blot analysis; C, secretion of IL‐6, IL‐1β and TNF‐α in mouse lung tissue homogenate determined by ELISA kits; D, number of apoptotic epithelial cells determined by TUNEL; E, histopathologic changes in mouse lung tissues observed through HE staining F, lung fibrosis in mouse lung tissues determined by Masson's trichrome staining. Each spot in the images indicates one sample. N = 8 in each group. Three independent experiments were performed. Data were expressed as mean ± SD compared by the unpaired t test. *P < .05, **P < .01