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. 2020 Apr 17;287(23):5218–5235. doi: 10.1111/febs.15311

Fig. 5.

Fig. 5

MDSCs enhance EOC stemness via CSF2. (A) qRT‐PCR analysis of CSF2 expression in ES2 and SKOV3 cells transfected with the shControl or shCSF2 plasmid. (B) Western blot analysis of CSF2 expression in ES2 and SKOV3 cells transfected with the ShControl or ShCSF2 plasmid. (C, D) Sphere formation assays with ES2 and SKOV3 cells with dysregulated CSF2 expression cultured with or without MDSCs. Scale bar, 50 μm. (E, F) Colony formation assays with ES2 and SKOV3 cells with knocked down CSF2 expression cultured with or without MDSCs. (G, H) Flow cytometry analysis of the ALDH+ cells in ES2 and SKOV3 cell populations transfected with the shControl or shCSF2 plasmid and cultured with MDSCs. All data were analysed using Student’s t‐test and are expressed as the mean ± SD. Experiments were performed in triplicate with MDSCs derived from three different patients. Symbols represent statistical significance (*P < 0.01; **P < 0.001; and ***P < 0.0001; ns, P> 0.05).