Figure 8.

Protective effect of docosahexaenoic acid 22:6 n‐3 (DHA) in the presence of ZVAD and chloroquine in NGFDPC12 cells. NGFDPC12 cells were treated with BSA control medium (CTL), DHA, palmitic acid (PAM), and PAM + DHA in the presence or absence of ZVAD at 40 µM (a) and chloroquine (CQ, autophagy inhibitor, 20 µM) (b) for 48 hr. Crystal violet assay was performed to determine cell viability. Results are expressed as percent of control and shown as Mean ± SEM, n = 7–8 (a) and n = 4–5 (b) of independent cell culture preparations. Statistical analysis was done by one‐way ANOVA followed by multiple comparison test. Significance symbols: **p < .01; ****p < .0001. (c) Representative microscopic images show the morphology of NGFDPC12 cells 48 hr after treatments