FIGURE 5.

ALA maintains a low ROS level and inhibits apoptosis of hematopoietic stem/progenitor cells. (A) Representative flow cytometry results of ROS levels in hematopoietic stem/progenitor cells from normal or ALA‐treated cocultures on day 14. (B) Mean fluorescence intensity (MFI) of ROS (DCFH‐DA) was calculated. (C) Representative flow cytometry results of apoptosis of hematopoietic stem/progenitor cells from normal and ALA‐treated cocultures on day 14. (D) The percentages of live cells, early apoptosis (E‐apoptosis), late apoptosis (L‐apoptosis), and cell death were calculated. (E) RT‐PCR indicated the mRNA encoding relative expression levels of stem cell maintenance or cell apoptosis related genes in hematopoietic stem/progenitor cells, the data were presented as –△Ct (Ct _B2M – Ct _Genes) value. (F and G) Intracellular staining assay of was performed to analysis the protein expression level of HIF1A (F) and P53 (G) in CD34⁺CD43⁺CD45^+/low cells at day14 of coculture. Data in the bar graphs in (B) and (D–F) are presented as the mean ± sd. An unpaired Student's t‐test (2‐tailed) was performed. N = 3 replicates; ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001, ^**** P < 0.0001; ns, not significant