Skip to main content
. Author manuscript; available in PMC: 2020 Dec 22.
Published in final edited form as: Immunohorizons. 2020 Sep 21;4(9):561–572. doi: 10.4049/immunohorizons.2000075

FIGURE 2. R837 induces inflammatory cytokines in adult mouse CMs via a TLR7-dependent mechanism.

FIGURE 2.

Cell culture medium was changed to prewarmed, serum-free M199 medium containing 0.05% BSA for 1 h prior to treatment. CMs were treated with R837 and LPS at the specified doses for 16–18 h before the media was collected. (A) Time course of CXCL2 production after treatment with 1.25 μg/ml R837. (B) Dose-response curves of R837 in WT and TLR7 KO CMs. (C) Cytokine production in WT and TLR7 KO CMs treated with 1.25 μg/ml R837 or 500 ng/ml LPS. Each error bar represents mean ± SD. Time course in (A) was analyzed with one-way ANOVA with Tukey post hoc test. Dose curves in (B) were analyzed with multiple two-tailed t tests. Data in (C) were analyzed with two-way ANOVA with Tukey post hoc test. Experiments were performed in triplicates and repeated independently two to three times. **p < 0.01, ***p < 0.001, ****p < 0.0001.