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. Author manuscript; available in PMC: 2020 Dec 22.
Published in final edited form as: Immunohorizons. 2020 Sep 21;4(9):561–572. doi: 10.4049/immunohorizons.2000075

FIGURE 3. Intracardiac injection of miR-146a-5p results in TLR7-dependent cytokine production in the heart.

FIGURE 3.

(A) Sixty microliters of phthalo blue dye was injected into the anterior wall of left ventricle to outline the area of injection. Hearts were sectioned into four slices and imaged on both sides to show injection primarily stays in the myocardium. (B) miR-146a-5p expression in the heart 24 h after injection of Lipo or miR-146a-5p in WT and TLR7 KO mice. (C–E) Quantitative RT-PCR analysis of cytokine genes in the hearts injected with Lipo or miR-146–5p. Each error bar represents mean ± SD (n = 6 mice in each group). Data in (B) were analyzed using a Mann-Whitney nonparametric test as the data did not pass the normality test. Data in (C)–(E) were analyzed with two-way ANOVA with Tukey post hoc test. *p < 0.05, **p < 0.01. Lipo, lipofectamine; miR-146, miR-146a-5p.